• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Electrochemiluminescence immunoassay system and a flow cell unit (1) thereof are used for an electrochemiluminescence reaction to occur in the flow cell unit (1) for a liquid to be tested, and the flow cell unit (1) comprises a working electrode (12), a counter electrode (11) and a reference electrode (13), wherein the working electrode (12) and the counter electrode (11) are provided vertically and there is a liquid flow path between the two electrodes. When an electrochemical reaction occurs and the reactants are evenly distributed on the working electrode (12) for a test, it is convenient to control the reactants, and after the flow cell is cleaned, the liquid flow path is cleared so that the cleaning effect is better, which is one can avoid electrode aging caused by poor cleaning, which is advantageous for the repeated use of the flow cell unit (1) and considerably improves the accuracy of a measurement result. A porous structure is provided on a connection surface, the reference electrode (13) communicating with the liquid flow path and the porous structure of the reference electrode (13) providing good electrical conductivity and preventing premature aging of the reference electrode (13) and the durability of the flow cells -Unit (1) improved provided that the functions of the reference electrode (13) are guaranteed.
    公开号:CH713287B1
    申请号:CH00550/18
    申请日:2016-03-28
    公开日:2019-12-30
    发明作者:Qin Jun
    申请人:Beijing Unidiag Tech Inc；
    IPC主号:
    专利说明:

    description
    Technical Field of the Invention The present invention relates to the field of medical device technology, particularly to an electrochemiluminescent immunoassay system and a flow cell unit thereof.
    Background Art ECL (Electrochemoluminescence) refers to a chemical reaction generated by the reaction products on the electrode surface or between the reaction products on the electrode surface and some components in the system after a constant voltage has been applied to the electrode. The reaction process comprises three stages: an electrochemical reaction process, chemiluminescence and a cycle process.
    [0003] Electrochemiluminescence immunoassay technology is a combination of electrochemiluminescence (ECL) and immunoassay. The principle of the luminescence of the marker differs from that of regular chemiluminescence (CL). It is a kind of specific chemiluminescence reaction that is triggered by an electrochemical reaction on the electrode surface, which actually comprises two processes: electrochemistry and chemiluminescence. The difference between ECL and CL is that the ECL is a luminescence reaction that is triggered by electricity, while CL is a luminescence reaction that is triggered by the mixed compounds.
    [0004] The ECL can not only be used in all immunoassays, but can also be used in DNA / RNA studies. It is a new generation of labeled immunoassay technology after the appearance of the radioimmunoassays (RIA), the enzyme immunoassays (EIA), the fluorescence immunoassays (FIA), and the chemiluminescence immunoassays. ECL technology is adapted to the principle of many types of immune reactions, and its unique advantage enables the development of a large number of detection reagents based on this platform for more than 100 products such as hormones, thyroid function, tumor markers, myocardial markers, anemia, infectious diseases and many others.
    The container of the conventional electrochemiluminescence detector in which the electrochemiluminescence reaction takes place currently mainly comprises three types, namely the reaction cell, the reaction cup of a printed electrode and a flow cell.
    First, for the electrochemiluminescence detector whose electrochemical reaction takes place in the reaction cell, the reaction cell is not easy to clean and reuse. In addition, the reaction electrode, the counter electrode and the reference electrode of the detector are generally columnar, which is suitable for laboratory tests with a small amount of assays, but not for large-scale clinical assays.
    Second, in the electrochemiluminescence detector, in which three electrodes are printed in the reaction vessel, the electrochemiluminescence reaction takes place on the three electrodes in the reaction vessel. The reaction cup can only be used once, and the disposable costs are too high due to the relatively high value of the electrode material. In addition, the reference electrode should be immersed in liquid before and during use to serve as a reference. The reaction cup of the printed electrode exposes the reference electrode to the dry air and can therefore tend to oxidize or age.
    Finally, in a flow cell associated with a conventional electrochemiluminescence detector, the working electrode of the flow cell is placed in the center of the electrode trough and the counter electrode surrounds the working electrode on the periphery. These two electrodes are on the same horizontal plane. When cleaning a flow cell with this structure, the cleaning liquid must flow around the gap between the working and counter electrodes. Cleaning is difficult and the cleaning result is not good, which means that the two electrodes can age easily, which affects the accuracy of the measurement result and reduces the life of the flow cell.
    In view of the disadvantages of the above flow cell, there is an urgent need to provide a reusable flow cell, the electrode of which is not easy to age and which gives accurate measurement results.
    Summary of the Invention In order to solve the above technical problems, the first object of the present disclosure is to provide a flow cell unit for an electrochemiluminescent immunoassay system. In the flow cell unit, the counter electrode and the working electrode are arranged one above the other, which is suitable for cleaning the flow cell and has no blind spot, as a result of which aging of the electrode due to poor cleaning can be avoided and thus the accuracy of the measurement results is drastically improved.
    [0011] The second object of the present disclosure is to provide an electrochemiluminescent immunoassay system that includes the flow cell assembly.
    CH 713 287 B1 In order to achieve the first object of the present disclosure, the present disclosure represents a flow cell unit for an electrochemiluminescent immunoassay system in which a test liquid undergoes an electrochemiluminescent reaction which has the features of claim 1.
    [0013] In the present disclosure, the working and counter electrodes are arranged one above the other. When the flow cell is cleaned, the cleaning liquid does not have to flow around the two electrodes, so that it can be cleaned comfortably and without a blind spot, so that a better cleaning result is achieved, so that the aging of the electrode is avoided by poor cleaning and so that the accuracy of what can be achieved Measurement result is significantly improved.
    Optionally, the counter electrode consists of two needle electrodes arranged above the working electrode and the working electrode is a surface electrode.
    [0015] Connected screw holes are optionally provided in the top plate, the gasket and the bottom plate.
    Optionally, at least one end of the first working section bends in the direction of the upper plate to a first hook, a first hook groove corresponding to the first hook is provided in the upper plate and the first hook is clipped into the first hook groove; wherein at least one end of the second working section bends in the direction of the lower plate to form a second hook, a second through hole corresponding to the second hook being provided on the lower plate, and the second hook being clipped onto the second through hole.
    To achieve the second objective of the present disclosure, the present disclosure provides an electrochemiluminescent immunoassay system that includes a flow cell unit and a detection unit for detecting the flow cell unit, and further a control unit for controlling the operation the flow cell unit and a mounting plate for fixing the flow cell unit and the control unit, wherein the flow cell unit is the above-mentioned flow cell unit.
    Optionally, the control unit consists of a rotating arm and a stepper motor; one end of the pivot arm is hinged to the stepper motor to control the rotation of the pivot arm; and the other end of the rotary arm is connected to a magnet to attract the magnetic beads, also called magnetic beads, in the test liquid to the working electrode.
    Brief Description of the Drawings [0019]
    1 shows a perspective view of the flow cell unit,
    FIG. 2 shows a front view of the flow cell unit according to FIG. 1,
    3 shows a bottom view of the flow cell unit according to FIG. 1,
    FIG. 4 shows a perspective illustration according to FIG. 1 after the upper plate has been removed,
    5 shows a cross-sectional view of the reference electrode according to FIG. 1,
    Fig. 6 shows a cross-sectional view of Fig. 2 in the direction of line A-A,
    Fig. 7 shows a cross-sectional view of Fig. 2 in the direction of line B-B,
    8 shows a perspective view of the electrochemiluminescence immunoassay system according to the present disclosure, and
    FIG. 9 shows a front view according to FIG. 8.
    In Figures 1 to 9:
    I flow cell unit,
    II counter electrode,
    III first stage of work,
    112 first hook,
    Working electrode,
    CH 713 287 B1
    121 second hook,
    122 second work area,
    Reference electrode,
    131 housing of the reference electrode,
    132 third working section, upper plate, lower plate,
    151 second through hole;
    Poetry,
    161 first through hole,
    162 arch structure,
    groove;
    Controller,
    rotary arm,
    Stepper motor,
    Magnet,
    Detection unit
    Mounting plate.
    Detailed Description of Embodiments In order to assist those skilled in the art to better understand the technical solution of the present disclosure, the present disclosure is further explained with reference to the accompanying figures and examples.
    Reference is made to FIGS. 1 to 6, FIG. 1 being the perspective or structural representation of the flow cell unit; Figure 2 is a front view of Figure 1 and Figure 3 is a bottom view of Figure 1; Fig. 4 is a perspective or structural illustration of Fig. 1 after the top plate has been removed; 6 is a cross-sectional view of FIG. 2 in the direction of line A-A, and FIG. 7 is a cross-sectional view of FIG. 2 in the direction of line B-B.
    [0023] In one specific example, the present disclosure provides a flow cell unit 1 for the electrochemiluminescent immune system. The test liquid, which contains an antibody-antigen-luminescent agent coated with magnetic beads, is subjected to an electrochemiluminescent reaction in the flow cell unit 1. The flow cell unit consists of a working electrode 12 and a counter electrode 11, the working electrode 12 and the counter electrode 11 being arranged one above the other. In addition, the flow cell unit 1 also comprises a reference electrode 13, also called a reference electrode, which together with the working electrode 12 and the counter electrode 11 forms a triple electrode system. The working electrode 12 and the counter electrode 11 form a reaction circuit, and the working electrode 12 and the reference electrode 13 form a detection circuit.
    In the present example, the working electrode 12 and the counter electrode 11 are arranged one above the other. When the flow cell unit is cleaned, the cleaning liquid does not have to flow around the two electrodes, which is easier to clean and has no blind spot, so that the cleaning effect is better, which in turn prevents the electrode from aging due to poor cleaning, the life of the flow cells -Unit lengthened and the accuracy of the measurement result is significantly improved. Due to the stacked working electrode 12 and counter electrode 11, the components in the flow cell unit are arranged tightly packed, which reduces the space requirement and lowers the production costs.
    Specifically, the counter electrode 11, as shown in Fig. 4, consists of two needle electrodes, which are arranged above the working electrode 12, which is a flat electrode or surface electrode. During operation, the two needle electrodes apply the voltage at the same time. Together form a cathode for the electrochemiluminescence reaction and form a reaction circuit with the working electrode 12, so that the current of the working electrode 12 is continuous
    CH 713 287 B1 and the occurrence and recurrence of the electrochemiluminescent reaction on the working electrode 12 is ensured without influencing the reaction on the working electrode 12.
    In the present example, the working electrode 12 is a rectangular surface electrode with a smooth surface. When test liquid flows through the magnetic beads coated with antibody-antigen-luminescent agent, more test liquid can be applied to the surface of the working electrode 12 and distributed evenly. When the electrochemiluminescent reaction takes place, the efficiency of the electrochemiluminescent reaction is improved. If the detection occurs after the reaction, the evenly distributed analyte is easy to check, which improves the accuracy of the measurement result.
    At the same time, the counter electrode 11 is a needle electrode, which is connected to an overlying photomultiplier, through which the photons generated on the working electrode 12 are measured. Therefore, the needle-shaped counter electrode 11 can avoid blocking the optical path between the working electrode 12 and the photomultiplier tube and thus ensure the measurement accuracy. In order to ensure a sufficient area of the counter electrode 11, the counter electrode 11 also has two interacting needle electrodes.
    In addition, the counter electrode 11 and the working electrode 12 are arranged within the flow cell, which has a flow path for the liquid. The reference electrode 13 is arranged on one side of the flow cell, the working electrode 12, the counter electrode 11 and the reference electrode 13 being in fluid communication via an intermediate liquid flow path, and the reference electrode 13 having a third working section 132, which is in fluid communication with the liquid flow path stands and whose communication side with the liquid flow path is provided with a porous structure that prevents the liquid with column-base components in the liquid path from eroding the reference electrode 13 under the condition of the signal connection.
    The third working section 132 is held in a housing 131 of the reference electrode. In addition, a conductive solution such as potassium chloride or sodium chloride is also contained in the housing 131 of the reference electrode. In order to prevent oxidation of the third working section 132 by long-term use, the third working section 132 must be immersed in the conductive solution for a long time. In order to ensure that the third working section can be immersed in the conductive solution if there is less conductive solution in the housing 131 of the reference electrode, the third working section 132 is spiral-shaped (see FIG. 5) or it is an electrode with a cross section made of waveform , Triangle or other formats are provided so that the oxidation of the third working section 132 is effectively prevented, which makes the reference value stable and reliable during the assay and further improves the accuracy of a measurement result and increases the service life of the flow cell unit.
    In practice, the structures of the working electrode 12, the counter electrode 11 and the reference electrode 13 are not limited to the shapes and designs mentioned above. The cross section of the working electrode 12 can also have any other geometric shapes such as a circle or polygon. The cross-section of the counter electrode 11 can also be any other geometric shapes such as a surface or spiral, and the third working section 132 of the reference electrode 13 can be like a conventional needle electrode known from the prior art. Starting from the goal of improving the efficiency of the electrochemiluminescent reaction without affecting the signal detection and preventing the electrode oxidation, the structures of the working electrode 12, the counter electrode 11 and the reference electrode 13 are preferred as the structures in the example.
    In addition, the working electrode 12, the counter electrode 11 and the reference electrode 13 can be made of materials such as gold, platinum, graphite, silver, silver chloride, etc. Since platinum has a relatively good ductility and can be processed into various shapes, platinum electrodes are preferred in the present embodiment in order to improve the flexibility of the electrode structures. The porous structure at the interface between the third working section 132 and the liquid flow path may be a conventional porous structure such as porous ceramic, etc.
    Furthermore, the flow cell unit 1, as shown in Figs. 1 to 6, consists of an upper plate 14 and a lower plate 15, which are designed to match and form the flow cell unit. The upper plate 14 consists of transparent optical glass through which the light emitted by the electrochemiluminescent reaction is transmitted. At the same time, the counter electrode 11 is attached to the upper plate 14 and the working electrode 12 to the lower plate 15. There is a vertical gap between the working electrode 12 and the counter electrode 11, which forms the liquid flow path between the working electrode 12 and the counter electrode 11.
    As shown in Fig. 4, the flow cell unit 1 further includes a gasket 16 attached to the surface of the working electrode 12 on which a first through hole 161 is provided. The first through bore 161, the lower plate 15 and the upper plate 14 form a reaction chamber which is sealed by the seal 16 and thus prevents leakage of liquid. At the same time, the first through-hole 161 has a spindle shape with a cross section of two small ends and a large, wide center. In addition, a liquid inlet and a liquid outlet are respectively arranged at both ends of the spindle-shaped first through-hole 161, and the test and rinsing liquid flow through the spindle-shaped reaction chamber.
    CH 713 287 B1 In the example, the spindle-shaped reaction chamber has a small volume, so that when the test and rinsing liquid flow through the reaction chamber, the liquid flows almost linearly and without swirling. Therefore, the test or test liquid can come into sufficient contact with the working electrode 12 and the counter electrode 11 during the reaction, which is helpful for a smooth course of the reaction. It is even more important that when the electrode is rinsed, the rinsing liquid flows in from the liquid inlet and flows out of the liquid outlet. The cleaning liquid flows almost straight into the reaction space and rinses without a blind spot, which effectively prevents aging of the electrode.
    The first through hole 161 of the seal 16 is not limited to a spindle-shaped structure with two small ends and a large wide center, but can also be a rectangular structure which has a uniform cross section along the flow direction. However, if the first through-hole 161 is spindle-shaped, the cross-sectional areas of the liquid inlet and the liquid outlet are smaller compared to other parts, so that the flow rates at the liquid inlet and at the liquid outlet increase and the test liquid and the rinsing liquid flow quickly into and out of the reaction space.
    As shown in Fig. 4, the top plate 14, the gasket 16 and the bottom plate 15 have a plurality of screw holes which cooperate with screws or bolts. Therein, some bolts or screws connect the upper plate 14, the seal 16 and the lower plate 15 to the flow cell unit 1. Some other bolts or screws connect the flow cell unit 1 and the detection unit 3 arranged above the flow cell unit 1. The detection unit 3 measures the light passing through the upper plate 14.
    In addition, as shown in Fig. 4, the center of the seal 16 is a spindle-shaped structure, in the center of which the spindle-shaped first through-hole 161 is provided. The outer peripheral wall of the first through hole 161 protrudes outward and forms a plurality of arch structures 162 in which the screw holes are provided. In the example shown in FIG. 1, four screw holes are provided on the upper plate 14, the lower plate 15 and the seal 16. Two of them are used to connect the upper plate 14, the lower plate 15 and the gasket, and the other two are used to connect the flow cell unit 1 to the detection unit 3. In practice, the number of screw holes and the position is not limited to the number mentioned here, but can be set as required and is therefore not limited by the example here.
    In addition, the shape of the seal 16 is not limited to this, which can be coordinated with the rounding of the upper plate 14 and the lower plate 15. Therein, the spindle-shaped first through hole 161 is in the middle of the round structure and a plurality of screw holes are provided on two sides of the first through hole 161. However, in the example, the seal 16 requires the least material, and more importantly, it minimizes the light absorption by the seal 16, so that the accuracy of the measurement results is improved.
    On the other hand, as shown in Fig. 4, the counter electrode 11 comprises a first working section 111, the working electrode 12 a second working section 122 and the first working section 111 and the second working section 122 form a reaction circuit. In addition, the area of the second working section 122 is smaller than that of the first through hole 161. This setting further reduces the size of the components in the flow cell unit 1, which contributes to the miniaturization of the device.
    The first working section 111 is meanwhile connected to a first hook 112 which extends in the direction of the upper plate 14. Accordingly, a first hook groove matching the first hook 112 is placed on the upper plate 14. The first hook 112 is clipped into the first hook groove so that the counter electrode 11 is attached to the upper plate 14.
    In particular, as shown in FIGS. 4 and 5, two ends of the first working section 111 bend in the direction of the upper plate 14 and form two first hooks 112. On the upper plate 14 there are two first hook grooves matching the first hook 112 set. The first hook 112 is an inverse hook of the "L" type, and accordingly the first hook groove is an inverse hook groove of the "L" type, which corresponds to the inverse hook of the "L" type.
    Since the counter electrode 11 is two needle electrodes, the material of which is usually platinum, it can be bent and folded into a variety of shapes. When these are attached to the upper plate 14, the two ends are bent into an inverse hook of the “L” type, as a result of which the counterelectrode 11 is firmly connected to the upper plate 14, so that the stability of the electrochemiluminescent reaction is ensured.
    Furthermore, as shown in Fig. 2, a groove is provided on the outer circumference of the upper plate 14 and the lower plate 15. The first two hook grooves extend up to the top of the top plate 14, and each of the first two hook grooves extends to the groove 17 so that one end of the counter electrode 11 extends to the groove 17 through which the counter electrode 11 has an outer Voltage and current source comes into contact, which applies a corresponding voltage to the counter electrode 11.
    In the example shown in FIGS. 4 and 6, the working electrode 12 is perpendicular to the first through hole 161 of the seal 16. The second working section 122 bends the direction of the lower plate 15 downward and forms a second hook 121 a second through hole 151 is provided on the lower plate 15 to match the second hook 121, and the second hook 121 is clipped onto the second through hole 151 so that
    CH 713 287 B1 the working electrode 12 is attached to the top of the lower plate 15. In addition, through the second through hole 151, the second hook 121 contacts the external power source, which supplies the working electrode 12 with voltage.
    It is believed that the shape of the first hook 112 and the second hook 121 is not limited to the shapes specified herein, and any other conventional form of the prior art can be used as long as it is the connection between the counter electrode 11 and the upper plate 14 and the counter electrode 12 and the lower plate 15 realized. In practice, the connections between the counter electrode 11 and the upper plate 14 and the working electrode 12 and the lower plate 15 are not limited to clipping on, but can be any other type of connection and connection. So there is no restriction on the type and shape of the connection and on the position of the two hooks.
    In addition, the lower plate 15 and the housing 131 of the reference electrode in the example are made of corrosion-resistant peek material to protect the electrodes.
    Referring to FIGS. 8 and 9, FIG. 8 shows a perspective view of the electrochemiluminescent immunoassay system of the present disclosure, and FIG. 9 shows a front view of FIG. 8.
    As shown in Figs. 8 and 9, the present disclosure is an electrochemiluminescent immunoassay system that includes the flow cell unit 1 and a detection unit 3 for analyzing the flow cell unit 1, which also includes a control unit 2 for controlling the flow cells Unit 1 and a fixed base 4 for fastening the flow cell unit 1 and the control unit 2, wherein the flow cell unit 1 is the flow cell unit 1 mentioned in one of the above examples. Because the flow cell unit 1 has the above technical effects. the electrochemiluminescence immunoassay system comprising the flow cell unit 1 also has the same technical effects which need not be repeated here.
    The control unit, as shown in FIGS. 7 and 8, consists of a rotating arm 21, one end of the rotating arm 21 being connected to a stepping motor 22 via a hinge and the other end of the rotating arm 21 being connected to a magnet 23 , Accordingly, a groove is provided on the underside of the lower plate of the flow cell unit 1. When the rotary arm 21 rotates into the groove under the control of the stepping motor 22, the magnet 23 moves into the groove, under the working electrode 12, and manipulates the magnetic beads in the test liquid to be attached to the working electrode 12 to trigger the electrochemiluminescent reaction. In addition, the magnet 23 is a permanent magnet in order to ensure a sufficient number of magnetic beads on the working electrode 12.
    In practice, the control device 2 does not have to be realized by the stepper motor 22 for controlling the rotary arm 21, but can in practice also be a conventional crank rod drive. The control unit 2 in the example, however, can strictly control the movement path of the rotary arm 21 by the stepping motor 22 and thus improve the accuracy of the device.
    When the electrochemiluminescence immunoassay system is working, the rotary arm 21 is first rotated under the control of the stepping motor 22 of the control unit 2 to the underside of the working electrode 12; the test liquid is attached and provided by the magnet 23 on the rotating arm 21 on the surface of the flat working electrode 12; the one with a marker, i.e. Ruthenium, bound complex is separated from the free marker under the action of a magnetic field. In the meantime, a solution with TPA or DBAE (dibutylaminoethanol) is added and voltage is applied to start the ECL reaction. Luminescent substrate pyridine-ruthenium (II) and TPA lose an electron and are oxidized on the surface of the working electrode 12 to pyridine-ruthenium (III) and cation-excited state TPA +. At the same time, the cation-excited state TPA + removes a proton and becomes an excited TPA with a strong reduction ability. The pyridine-ruthenium (III) with strong oxidizability and the excited state TPA with strong reducibility undergo an oxidation-reduction reaction, so that the pyridine-ruthenium (III) is reduced to excited pyridine-ruthenium (II). The excited pyridine-ruthenium (II) decays in a fluorescent mechanism, releases energy by emitting a 620nm photon and becomes the ground state of the luminescent substrate pyridine-ruthenium (II).
    This process is repeated on the surface of the electrode and generates many photons. The light intensity is measured by the photomultiplier or photoelectron multiplier tube in the detection unit 3, then amplified and analyzed by a computer. The light intensity is linearly linked to the concentration of pyridine ruthenium, and the concentration of the antigen in the test liquid is converted on the basis of the light intensity emitted by the pyridine ruthenium at the working electrode 12. The principle of the DBAE reaction system corresponds to that of the TPA reaction system described above.
    [0053] In the foregoing, an electrochemiluminescent immunoassay system has been presented, which is provided by the present disclosure, and a flow cell unit thereof has been described in detail. Concrete examples are used here to describe the principles and implementation modalities of the present disclosure, and the description of the example is only intended to help understand the method and principles of the present disclosure. It is to be understood that those skilled in the art can make various improvements and changes to the present disclosure without departing from the principle of the present invention, and such improvements and changes fall within the scope of the claims of the present invention.
    CH 713 287 B1
    权利要求:
    Claims (6)
    [1]
    claims
    1. flow cell unit of an electrochemiluminescence immunoassay system, in which a test liquid is subjected to an electrochemiluminescence reaction, comprising a working electrode (12) and a counter electrode (II), the working electrode (12) and the counter electrode (11) being arranged one above the other, wherein the counter electrode (11) and the working electrode (12) are arranged in a flow cell which has a liquid flow path for flowing them through; wherein the counter electrode (11) comprises a first working section (111), the working electrode (12) comprises a second working section (122), and the first working section (III) and the second working section (122) form a reaction cycle; the flow cell unit further comprising a reference electrode (13) disposed on one side of the flow cell, the working electrode (12), the counter electrode (11) and the reference electrode (13) in fluid communication with each other through the liquid flow path therebetween and the reference electrode (13) has a third working section (132) which is in fluid communication with the liquid flow path and the connection side of which is provided with a porous structure with the liquid flow path, the flow cell unit comprising an upper plate (14) and a lower one Plate (15) is formed, which are designed to match each other, wherein the upper plate (14) consists of a transparent material, the counter electrode (11) is attached to the upper plate (14) and the working electrode (12) on the lower plate (15) is fixed, wherein a seal (16) with a first through hole (161) between The upper plate (14) and the lower plate (15) is provided, wherein the first through hole (161), the lower plate (15) and the upper plate (14) form a reaction chamber, wherein a liquid inlet and a liquid outlet in the bottom plate (15) are provided under the reaction chamber and the test liquid undergoes an electrochemiluminescent reaction in the reaction chamber.
    [2]
    2. Flow cell unit according to claim 1, wherein the counter electrode (11) has two needle electrodes arranged above the working electrode (12) and the working electrode (12) is a flat electrode.
    [3]
    3. The flow cell unit according to claim 1, wherein communicating screw holes are provided in the upper plate (14), the gasket (16) and the lower plate (15).
    [4]
    4. The flow cell assembly of claim 1, wherein at least one end of the first working section (111) is bent toward the top plate (14) to form a first hook (112), a first corresponding to the first hook (112) Hook groove is provided in the top plate (14) and the first hook (112) is clipped into the first hook groove; wherein at least one end of the second working portion (122) is bent toward the lower plate (15) to form a second hook (121), with a second through hole (151) corresponding to the second hook (121) the lower plate (15) is provided, and wherein the second hook (121) is clipped onto the second through hole (151).
    [5]
    5. electrochemiluminescence immunoassay system, comprising a flow cell unit (1) according to one of claims 1 to 4, and a detection unit (3) for detecting the light intensity, and further comprising a control unit (2) for controlling the operation of the flow cell Unit (1), and a mounting plate (4) for fixing the flow cell unit (1) and the control unit (2).
    [6]
    The electrochemiluminescent immunoassay system according to claim 5, wherein the control unit (2) comprises a rotating arm (21) and a stepping motor (22), one end of the rotating arm (21) being articulated to the stepping motor (22) to rotate of the rotary arm (21), and wherein the other end of the rotary arm (21) is connected to a magnet (23) to control the attraction of magnetic beads in the test liquid to the working electrode (12).
    CH 713 287 B1

    CH 713 287 B1

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    JP2018534567A|2018-11-22|
    KR102127484B1|2020-06-30|
    US10976260B2|2021-04-13|
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    法律状态:
    优先权:
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